Z703028
Sigma
B Cell Protocols
| ISBN-10: | 1-58829347-5 |
| ISBN-13: | 978-1-58829347-3 |
Description
| General description | Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. This book is a diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods in B cell biology. These readily reproducible protocols follow the successful Methods in Molecular Biology series format. |
Properties
| publication info | H. Gu and K. Rajewsky, ed., Humana Press, 2004, 304 pp., hard cover |
Table Of Contents
| Table of Contents | 1. Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen 2. Characterization of B Cell Matruation in the Peripheral Immune System 3. Identification of B Cell Subsets: An exposition of 11- Color (Hi-D) FACS Methods 4. Analysis of B Cell Life Span and Homeostasis 5. Differentiation of B Lymphocytes From Hematopoietic Stem Cells 6. Analysis of lymphocyte Development and Function Usgin the RAG Deficient Blastocyst Complementation System 7. Conditional Gene Mutagenesis in B Lineage Cells 8. Analysis of the Germinal Center Reaction and In Vivo Long Lived Plasma Cells 9. T Cell Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences 10. Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors 11. Immunoglobulin Class Switching: In Vitro Induction and Analysis 12. In Vitro and In Vivo Assays of B Lymphocyte Migration 13. Analysis of Antigen Specific B Cell Memory Directly Ex Vivo 14. B Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization 15. Isolation of Lipid Rafts From B Lymphocytes 16. Molecular Single Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells 17. Analysis of B Cell Immune Tolerance Induction Using Transgenic Mice 18. Analysis of B Cell Signaling Using DT40 B Cell Line 19. B Cell Development and Pre-B-1 Cell Plasticity In Vitro |
